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研究助理教授

原晓非

  • 简历
  • 科研
  • 教学
  • 发表论著

原晓非,2005年毕业于东京大学,获工学博士学位,博士期间主要研究蛋白质内包生体高分子胶束的药物载体并获得专利。博士后的研究方向是基于微纳米材料的生物传感器。通过采用独特的表面修饰技术为西班牙Biokit. S.A. 生物技术公司研发了新型的免疫检测粒子,极大地提高了该公司相应产品的检测质量。在英国格拉斯哥大学深造期间,研究领域涉及有机化学,微加工,生物学,微生物学和环境科学的交叉学科,不仅为日本日产化学工业株式会社研发了用于单细胞分析的3D微流控芯片的制作技术并申请专利,也作为项目参与申请人和主导科研人员成功地结题了多个重大科研项目,并以第一作者在高水平国际期刊上发表科研成果。

 

目前任职

南方科技大学环境科学与工程学院,研究助理教授

 

教育背景

2002.10 - 2005.09:工学博士,东京大学,工学院,材料工程专业
2000.10 - 2002.09:工学硕士,东京大学,工学院,材料工程专业
2000.04 - 2000.09:研究助理,东京大学,工学院,材料工程专业
1998.10 - 2000.03:日语一级,横滨国际教育学院,专修日语
1992.09 - 1996.07:工学学士,天津大学,应用化学系,高分子化工专业

工作经历
2018.08 – 至今:南方科技大学,环境科学与工程学院,研究助理教授
2011.05 – 2018.08:格拉斯哥大学,生物电子工程学院,助理研究员
2005.10 – 2011.03:筑波大学,数理物质科学研究院,博士后研究员

主要参与的科研项目
[1]  Industrial Biotechnology Innovation Centre (IBioIC, Project-2016-152), Engineering microbial cell factories for industrial carotenoids biosynthesis, 2017.07-2018-10, project leader.
[2]  Natural Environment Research Council (NERC, NE/P003826/1), Stable Isotope Probing with Resonance Raman Cell Sorting to profile influence of ocean acidification on microbial carbon fixation, 2016.07-2017.07, project leader.
[3]  Engineering and Physical Sciences Research Council (EPSRC, EP/K038885/1), Reconstructing anaerobic synthetic communities to fuel bioenergy production using microfluidics, 2015.05-2016.06, project leader.
[4]  Nissan Chemical Industries, Ltd., Development of a continuous microfluidic – Raman sorting platform, 2013.10-2015.04, project leader.
[5]  Leverhulme Trust, Substrate control of cell shape and effect on gene expression, 2011.05-2013.04, project co-leaders.

研究领域
[1] 基于微流控和纳米材料的生物传感器的研发
[2] 环境水体中污染物和致病菌的监测与治理
[3] 微流控在环境微生物研究中的应用

 

期刊论文
Research ID: J-9581-2016
ORCid: 0000-0001-5395-9109
[1]  Xiaofei Yuan, Yanqing Song, Yizhi Song, Jiabao Xu, Yinhu Wu, Andrew Glidle, Maggie Cusack, Umer Z. Ijaz, Jonathan M. Cooper, Wei E. Huang, Huabing Yin*. Effect of laser irradiation on cell function and its implications in Raman spectroscopy. Applied and Environmental Microbiology, 2018, 84 (8), e02508-17. (SJR Q1, IF 3.81)
[2]  Xiaofei Yuan, Jillian M. Couto, Andrew Glidle, Yanqing Song, William Sloan*, Huabing Yin*. Single-cell microfluidics to study the effects of genome deletion on bacterial growth behavior. ACS Synthetic Biology, 2017, 6(12), 2219-2227. (SJR Q1, IF 5.38)
[3]  Kimberly C. Martin, Xiaofei Yuan, Gregory Stimac, Kieran Bannerman, Jamie Anderson, Chloe Roy, Fokion Glykofrydis, Huabing Yin, Jamie A. Davies*. Symmetry-breaking in branching epithelia: cells on micro-patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility. Journal of Anatomy, 2017, 230(6), 766–774. (SJR Q1, IF 2.18)
[4]  Elise Cachat, Weijia Liu, Kim. C. Martin, Xiaofei Yuan, Huabing Yin, Peter Hohenstein, Jamie A. Davies*. 2- and 3-dimensional synthetic large-scale de novo patterning by mammalian cells through phase separation. Scientific Reports, 2016, 6, 20664. (SJR Q1, IF 4.26)
[5]  Xiaofei Yuan, Mi Zhou, Julie E. Gough, Andrew Glidle, Huabing Yin*. A novel culture system for modulating single cell geometry in 3D. Acta Biomaterialia, 2015, 24 (15), 228-240. (SJR Q1, IF 6.32)
[6]  Mi Zhou, Xiaofei Yuan, Huabing Yin, Julie E. Gough*. Restoration of chondrocytic phenotype on a two-dimensional micropatterned surface. Biointerphases, 2015, 10, 011003. (SJR Q2, IF 2.60)
[7]  Chunyan Li, Andrew Glidle, Xiaofei Yuan, Zhixiong Hu, Ellie Pulleine, Jon Cooper, Wantai Yang,* Huabing Yin*. Creating ‘Living’ polymer surface to pattern biomolecules and cells on common plastics. Biomacromolecules, 2013, 14, 1278-1286. (SJR Q1, IF 5.25)
[8]  Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, YukioNagasaki*. High PEGylation efficiency of pentaethylenehexamine-end poly(ethyleneglycol) (mPEG-N6) for active-ester surface. Colloids and Surfaces B-Biointerfaces, 2012, 92(1), 25-29. (SJR Q1, IF 3.89)
[9]  Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, YukioNagasaki*. Development of a high-performance immunolatex based on "soft landing" antibody immobilization mechanism. Colloids and Surfaces B-Biointerfaces, 2012, 99(1), 45-52. (SJR Q1, IF 3.89)
[10] Masaki Kubota, Keitaro Yoshimoto, Xiaofei Yuan, Yukio Nagasaki*. Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain layer. Polymer Journal, 2011,1-4. (SJR Q4, IF 2.15)
[11] Atsushi Hirano, Yutaka Maeda, Xiaofei Yuan, Ryuuichi Ueki, Yosuke Miyazawa, Junichi Fujita, Takeshi Akasaka, Kentaro Shiraki*. Controlled dispersion and purification of protein–carbon nanotube conjugates using guanidine hydrochloride. Chemistry-A European Journal, 2010, 16, 12221-12228. (SJR Q1, IF 5.32)
[12] Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, YukioNagasaki*. Efficient inhibition of interfacial nonspecific interaction to create practically utilizable high ferritin-response immunolatex. Analytical Chemistry, 2009, 81(24), 10097-10105. (SJR Q1, IF 6.32)
[13] Xiaofei Yuan, KeitaroYoshimoto, YukioNagasaki*. High-performance immunolatex possessing a mixed-PEG/antibody co-immobilized surface: High sensitive ferritin immunodiagnostics. Analytical Chemistry, 2009, 81(4), 1549-1556. (SJR Q1, IF 6.32)
[14] Xiaofei Yuan, Michihiro Iijima, Motoi Oishi, Yukio Nagasaki*. Structure and activity assay of nanozymes prepared by the co-immobilization of practically useful enzymes and hydrophilic block copolymers on gold nanoparticles. Langmuir, 2008, 24(13), 6903-6909. (SJR Q1, IF 3.83)
[15] Xiaofei Yuan, Yuichi Yamasaki, Atsushi Harada, Kazunori Kataoka*. Characterization of stable lysozyme-entrapped polyion complex (PIC) micelles with crosslinked core by glutaraldehyde. Polymer, 2005, 46(18), 7749-7758. (SJR Q1, IF 3.68)
[16] Xiaofei Yuan, Atsushi Harada, Yuichi Yamasaki, Kazunori Kataoka*. Stabilization of lysozyme-incorporated polyion complex (PIC) micelles by the -end-derivatization of poly (ethylene glycol)-poly (,-aspartic acid) block copolymers with hydrophobic groups. Langmuir, 2005, 21(7), 2668-2674. (SJR Q1, IF 3.83)
[17] Montree Jaturanpiny, Atsushi Harada, Xiaofei Yuan, Kazunori Kataoka*. Preparation of bionano-reactor based on core-shell structured polyion complexs micelles entrapping trypsin in the core cross-linked with glutaraldehyde. Bioconjugate Chemistry, 2004, 15, 344-348. (SJR Q1, IF 4.82)

会议论文
[18] Xiaofei Yuan, Chim Yahua, Huabing Yin*. Linking cell shape, elasticity and fate: In vitro re-differentiation of chondrocytes. Optical Elastography and Tissue Biomechanics (89460M), San Francisco, CA, USA, 2014.02.1-02.2.

学术著作(章节)
[19] Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, Yukio Nagasaki, Biomaterials (ACS Symposium series), Chapter 13: Design of highly functional antiferritin-Immunolatex by hybridization of antiferritin/mixed-PEG polymers onto polystylene submicroparticles. ACS publications, 2010,1054, 243-258.
[20] Xiaofei Yuan, Yukio Nagasaki. Current Technologies of Development and Application of Enzyme,Chapter 6:Preparation of PEG/enzyme co-immobilized gold nanoparticles and their function. CMC Publishing Co., Ltd., 2006, 275-281. (in Japanese)

发明专利
[21] Kazunori Kataoka, Yuichi Yamasaki, Xiaofei Yuan, Atsushi Harada, Montree Jaturanpiny. Stabilized polymer micelles. 2004,12.09, Japan, WO2004105799.
[22] Yukio Nagasaki, Xiaofei Yuan, Keitaro Yoshimoto. Construction of immunolatex possessing antibody/mixed-poly(ethylene glycol) co-immobilized surface). patent # 2009-007846.
[23] Yukio Nagasaki, Xiaofei Yuan, Motoi Oishi, Michihiro Iijima. Poly(ethylene glycol)/glucose dehydrogenase co-immobilized gold surface. patent # 2008-010589.
[24] Huabing Yin, Xiaofei Yuan, Andrew Glidle. A hydrodynamic focusing method and related device. 2016, UK, WO201655646A1.

研究领域
[1] 基于微流控和纳米材料的生物传感器的研发
[2] 环境水体中污染物和致病菌的监测与治理
[3] 微流控在环境微生物研究中的应用

期刊论文
Research ID: J-9581-2016
ORCid: 0000-0001-5395-9109
[1]  Xiaofei Yuan, Yanqing Song, Yizhi Song, Jiabao Xu, Yinhu Wu, Andrew Glidle, Maggie Cusack, Umer Z. Ijaz, Jonathan M. Cooper, Wei E. Huang, Huabing Yin*. Effect of laser irradiation on cell function and its implications in Raman spectroscopy. Applied and Environmental Microbiology, 2018, 84 (8), e02508-17. (SJR Q1, IF 3.81)
[2]  Xiaofei Yuan, Jillian M. Couto, Andrew Glidle, Yanqing Song, William Sloan*, Huabing Yin*. Single-cell microfluidics to study the effects of genome deletion on bacterial growth behavior. ACS Synthetic Biology, 2017, 6(12), 2219-2227. (SJR Q1, IF 5.38)
[3]  Kimberly C. Martin, Xiaofei Yuan, Gregory Stimac, Kieran Bannerman, Jamie Anderson, Chloe Roy, Fokion Glykofrydis, Huabing Yin, Jamie A. Davies*. Symmetry-breaking in branching epithelia: cells on micro-patterns under flow challenge the hypothesis of positive feedback by a secreted autocrine inhibitor of motility. Journal of Anatomy, 2017, 230(6), 766–774. (SJR Q1, IF 2.18)
[4]  Elise Cachat, Weijia Liu, Kim. C. Martin, Xiaofei Yuan, Huabing Yin, Peter Hohenstein, Jamie A. Davies*. 2- and 3-dimensional synthetic large-scale de novo patterning by mammalian cells through phase separation. Scientific Reports, 2016, 6, 20664. (SJR Q1, IF 4.26)
[5]  Xiaofei Yuan, Mi Zhou, Julie E. Gough, Andrew Glidle, Huabing Yin*. A novel culture system for modulating single cell geometry in 3D. Acta Biomaterialia, 2015, 24 (15), 228-240. (SJR Q1, IF 6.32)
[6]  Mi Zhou, Xiaofei Yuan, Huabing Yin, Julie E. Gough*. Restoration of chondrocytic phenotype on a two-dimensional micropatterned surface. Biointerphases, 2015, 10, 011003. (SJR Q2, IF 2.60)
[7]  Chunyan Li, Andrew Glidle, Xiaofei Yuan, Zhixiong Hu, Ellie Pulleine, Jon Cooper, Wantai Yang,* Huabing Yin*. Creating ‘Living’ polymer surface to pattern biomolecules and cells on common plastics. Biomacromolecules, 2013, 14, 1278-1286. (SJR Q1, IF 5.25)
[8]  Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, YukioNagasaki*. High PEGylation efficiency of pentaethylenehexamine-end poly(ethyleneglycol) (mPEG-N6) for active-ester surface. Colloids and Surfaces B-Biointerfaces, 2012, 92(1), 25-29. (SJR Q1, IF 3.89)
[9]  Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, YukioNagasaki*. Development of a high-performance immunolatex based on "soft landing" antibody immobilization mechanism. Colloids and Surfaces B-Biointerfaces, 2012, 99(1), 45-52. (SJR Q1, IF 3.89)
[10] Masaki Kubota, Keitaro Yoshimoto, Xiaofei Yuan, Yukio Nagasaki*. Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain layer. Polymer Journal, 2011,1-4. (SJR Q4, IF 2.15)
[11] Atsushi Hirano, Yutaka Maeda, Xiaofei Yuan, Ryuuichi Ueki, Yosuke Miyazawa, Junichi Fujita, Takeshi Akasaka, Kentaro Shiraki*. Controlled dispersion and purification of protein–carbon nanotube conjugates using guanidine hydrochloride. Chemistry-A European Journal, 2010, 16, 12221-12228. (SJR Q1, IF 5.32)
[12] Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, YukioNagasaki*. Efficient inhibition of interfacial nonspecific interaction to create practically utilizable high ferritin-response immunolatex. Analytical Chemistry, 2009, 81(24), 10097-10105. (SJR Q1, IF 6.32)
[13] Xiaofei Yuan, KeitaroYoshimoto, YukioNagasaki*. High-performance immunolatex possessing a mixed-PEG/antibody co-immobilized surface: High sensitive ferritin immunodiagnostics. Analytical Chemistry, 2009, 81(4), 1549-1556. (SJR Q1, IF 6.32)
[14] Xiaofei Yuan, Michihiro Iijima, Motoi Oishi, Yukio Nagasaki*. Structure and activity assay of nanozymes prepared by the co-immobilization of practically useful enzymes and hydrophilic block copolymers on gold nanoparticles. Langmuir, 2008, 24(13), 6903-6909. (SJR Q1, IF 3.83)
[15] Xiaofei Yuan, Yuichi Yamasaki, Atsushi Harada, Kazunori Kataoka*. Characterization of stable lysozyme-entrapped polyion complex (PIC) micelles with crosslinked core by glutaraldehyde. Polymer, 2005, 46(18), 7749-7758. (SJR Q1, IF 3.68)
[16] Xiaofei Yuan, Atsushi Harada, Yuichi Yamasaki, Kazunori Kataoka*. Stabilization of lysozyme-incorporated polyion complex (PIC) micelles by the -end-derivatization of poly (ethylene glycol)-poly (,-aspartic acid) block copolymers with hydrophobic groups. Langmuir, 2005, 21(7), 2668-2674. (SJR Q1, IF 3.83)
[17] Montree Jaturanpiny, Atsushi Harada, Xiaofei Yuan, Kazunori Kataoka*. Preparation of bionano-reactor based on core-shell structured polyion complexs micelles entrapping trypsin in the core cross-linked with glutaraldehyde. Bioconjugate Chemistry, 2004, 15, 344-348. (SJR Q1, IF 4.82)

会议论文
[18] Xiaofei Yuan, Chim Yahua, Huabing Yin*. Linking cell shape, elasticity and fate: In vitro re-differentiation of chondrocytes. Optical Elastography and Tissue Biomechanics (89460M), San Francisco, CA, USA, 2014.02.1-02.2.

学术著作(章节)
[19] Xiaofei Yuan, Dolça Fabregat, KeitaroYoshimoto, Yukio Nagasaki, Biomaterials (ACS Symposium series), Chapter 13: Design of highly functional antiferritin-Immunolatex by hybridization of antiferritin/mixed-PEG polymers onto polystylene submicroparticles. ACS publications, 2010,1054, 243-258.
[20] Xiaofei Yuan, Yukio Nagasaki. Current Technologies of Development and Application of Enzyme,Chapter 6:Preparation of PEG/enzyme co-immobilized gold nanoparticles and their function. CMC Publishing Co., Ltd., 2006, 275-281. (in Japanese)

发明专利
[21] Kazunori Kataoka, Yuichi Yamasaki, Xiaofei Yuan, Atsushi Harada, Montree Jaturanpiny. Stabilized polymer micelles. 2004,12.09, Japan, WO2004105799.
[22] Yukio Nagasaki, Xiaofei Yuan, Keitaro Yoshimoto. Construction of immunolatex possessing antibody/mixed-poly(ethylene glycol) co-immobilized surface). patent # 2009-007846.
[23] Yukio Nagasaki, Xiaofei Yuan, Motoi Oishi, Michihiro Iijima. Poly(ethylene glycol)/glucose dehydrogenase co-immobilized gold surface. patent # 2008-010589.
[24] Huabing Yin, Xiaofei Yuan, Andrew Glidle. A hydrodynamic focusing method and related device. 2016, UK, WO201655646A1.